Estimation of the radical scavenging activity 2,2-diphenyl-1- picrylhydrazyl, total phenols and total flavonoids of Vitex agnuscastus L. methanolic leaf extract in Baghdad
Keywords:
Vitex agnus castus, Antioxidant, DPPH, Total phenols, Total FlavonoidsAbstract
Chaste tree, is a plant distributed in few regions like the Middle East and Southern Europe. Conventionally used for the treatment of several health issues and symptoms, such as hormonal changes premenstrual ones and spasmodic dysmenorrhea, certain menopausal conditions, poor lactation, and pimples (acne). Several reports have told that Vitex agnus-castus contained phytochemical that really interesting to be studied like phenols and flavonoids, and others. The work aims to estimate the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay activity of methanol extracts from leaves of Vitex agnus-castus in Iraq, and see if it contains appreciate level of bioactive ingredient of phenols and flavonoids. Dried samples were submitted to extraction with methanol using soxhlet and total flavonoids, and phenols were tested in the sample. DPPH free radical levels were significantly lowered in a concentration-dependent mode, there was no statistically significant difference (p >0.05) between our extract and ascorbic acid in percent of inhibition of free radical. The IC50 found to be 725.4 μg/ ml as compared with the standard ascorbic acid (246.53 μg/ml), and the phenols level was 43.43 ± 1.12 (mg GAE/g extract) mg of Gallic Acid Equivalents (GAE) per g of extract, while the flavonoid was 14.7 ± 0.25 (mg QE/g) mg quercetin equivalence per gram dried extract. Therefore, Iraqi Vitex agnus-castus L. can be considered as encouraging candidates for natural herb sources of antioxidants with high value.
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